Pharmacological modifications of the partial pressure of oxygen in murine tumors: evaluation using in vivo EPR oximetry

Magn Reson Med. 1999 Oct;42(4):627-30. doi: 10.1002/(sici)1522-2594(199910)42:4<627::aid-mrm2>3.0.co;2-m.

Abstract

EPR oximetry using an implantable paramagnetic probe was used to quantify the partial pressure of oxygen (pO(2)) in tissues in a transplantable mouse tumor model (TLT) after administration of 34 different vasodilators belonging to one of the following classes: angiotensin-converting enzyme inhibitors, calcium antagonists, alpha antagonists, potassium channel openers, beta-blockers, NO donors, and peripheral vasoactive agents. Twenty-four compounds were efficient in significantly increasing the local pO(2) in a majority of tumors. The increase of local pO(2) using pharmacological treatments was lower than that achieved by using oxygen or carbogen breathing. This technique offers an unprecedented tool for rapidly and accurately measuring treatment-induced modifications of pO(2) in tumors. Magn Reson Med 42:627-630, 1999.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Electron Spin Resonance Spectroscopy
  • Liver Neoplasms, Experimental / drug therapy*
  • Liver Neoplasms, Experimental / metabolism
  • Male
  • Mice
  • Mice, Inbred Strains
  • Neoplasm Transplantation
  • Oximetry
  • Oxygen / metabolism*
  • Partial Pressure
  • Vasodilator Agents / pharmacology*

Substances

  • Vasodilator Agents
  • Oxygen