Rapid identification of Campylobacter, Arcobacter, and Helicobacter isolates by PCR-restriction fragment length polymorphism analysis of the 16S rRNA gene

S M Marshall; P L Melito; D L Woodward; W M Johnson; F G Rodgers; M R Mulvey

doi:10.1128/JCM.37.12.4158-4160.1999

Rapid identification of Campylobacter, Arcobacter, and Helicobacter isolates by PCR-restriction fragment length polymorphism analysis of the 16S rRNA gene

J Clin Microbiol. 1999 Dec;37(12):4158-60. doi: 10.1128/JCM.37.12.4158-4160.1999.

Authors

S M Marshall¹, P L Melito, D L Woodward, W M Johnson, F G Rodgers, M R Mulvey

Affiliation

¹ National Research Council Canada, Institute for Biological Sciences, Ottawa, Ontario K1A 0R6, Canada.

Abstract

A rapid two-step identification scheme based on PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the 16S rRNA gene was developed in order to differentiate isolates belonging to the Campylobacter, Arcobacter, and Helicobacter genera. For 158 isolates (26 reference cultures and 132 clinical isolates), specific RFLP patterns were obtained and species were successfully identified by this assay.

MeSH terms

Arcobacter / classification*
Arcobacter / genetics
Campylobacter / classification*
Campylobacter / genetics
Campylobacter Infections / microbiology
Genes, Bacterial
Genes, rRNA*
Gram-Negative Bacterial Infections / microbiology
Helicobacter / classification*
Helicobacter / genetics
Helicobacter Infections / microbiology
Humans
Polymerase Chain Reaction*
Polymorphism, Restriction Fragment Length*
RNA, Ribosomal, 16S / genetics

Substances

RNA, Ribosomal, 16S