Epidermal growth factor regulation in adult rat alveolar type II cells of amiloride-sensitive cation channels

Am J Physiol. 1999 Dec;277(6):C1058-65. doi: 10.1152/ajpcell.1999.277.6.C1058.

Abstract

Using the patch-clamp technique, we studied the effects of epidermal growth factor (EGF) on whole cell and single channel currents in adult rat alveolar epithelial type II cells in primary culture in the presence or absence of EGF for 48 h. In symmetrical sodium isethionate solutions, EGF exposure caused a significant increase in the type II cell whole cell conductance. Amiloride (10 microM) produced approximately 20-30% inhibition of the whole cell conductance in both the presence and absence of EGF, such that EGF caused the magnitude of the amiloride-sensitive component to more than double. Northern analysis showed that alpha-, beta- and gamma-subunits of rat epithelial Na(+) channel (rENaC) steady-state mRNA levels were all significantly decreased by EGF. At the single channel level, all active inside-out patches demonstrated only 25-pS channels that were amiloride sensitive and relatively nonselective for cations (P(Na(+))/P(K(+)) approximately 1.0:0.48). Although the biophysical characteristics (conductance, open-state probability, and selectivity) of the channels from EGF-treated and untreated cells were essentially identical, channel density was increased by EGF; the modal channel per patch was increased from 1 to 2. These findings indicate that EGF increases expression of nonselective, amiloride-sensitive cation channels in adult alveolar epithelial type II cells. The contribution of rENaC to the total EGF-dependent cation current under these conditions is quantitatively less important than that of the nonselective cation channels in these cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Age Factors
  • Amiloride / pharmacology
  • Animals
  • Blotting, Northern
  • Cell Separation
  • Cell Survival
  • Cells, Cultured
  • Diuretics / pharmacology
  • Electric Conductivity
  • Epidermal Growth Factor / pharmacology*
  • Epithelial Sodium Channels
  • Ion Channel Gating / drug effects
  • Ion Channel Gating / physiology
  • Male
  • Membrane Potentials / drug effects
  • Membrane Potentials / physiology
  • Patch-Clamp Techniques
  • Pulmonary Alveoli / chemistry
  • Pulmonary Alveoli / cytology*
  • Pulmonary Alveoli / drug effects
  • RNA, Messenger / analysis
  • Rats
  • Rats, Sprague-Dawley
  • Sodium / metabolism
  • Sodium Channels / genetics
  • Sodium Channels / metabolism*
  • Up-Regulation / physiology

Substances

  • Diuretics
  • Epithelial Sodium Channels
  • RNA, Messenger
  • Sodium Channels
  • Epidermal Growth Factor
  • Amiloride
  • Sodium