HMG17, a member of the high-mobility group of proteins, is ubiquitously expressed in higher eukaryotes. This protein has been shown to enhance transcriptional activity of many other genes. Recently, the HMG17 gene has been mapped to chromosomal band 1p36.1. Because this region is frequently involved in chromosomal aberrations of various human neoplasms, two PAC clones containing an HMG17 sequence were isolated. By fluorescence in situ hybridization (FISH) on prometaphase spreads, HMG17 was mapped to chromosomal band 1p35.