Significance of alpha-2-macroglobulin, alpha-1-acid glycoprotein, and C-reactive protein in pleural effusion differentiation

Respiration. 2000;67(1):30-5. doi: 10.1159/000029459.

Abstract

Background: The differentiation between exudates and transudates is fundamental when investigating the cause of pleural effusions. Acute-phase proteins could be potentially useful markers in this discrimination.

Objective: The present study was designed to evaluate whether the acute-phase proteins: alpha(2)-macroglobulin (AMG), alpha(1)-acid glycoprotein (AAG) and C-reactive protein (CRP) are useful in investigating the pleural effusions.

Methods: We prospectively measured the concentrations of the above proteins in the serum and pleural fluid of 84 consecutive patients with various diseases using a nephelometric assay.

Results: Pleural effusion AMG, AAG and CRP were all significantly elevated in the group of patients with exudates compared to patients with transudates (p < 0.001, p < 0.001 and p < 0.01, respectively). An AAG value >63 mg/dl in a pleural effusion is predictive of an exudate with a sensitivity of 90% and a specificity 85%. Similarly, an AMG value >44 mg/dl in a pleural effusion is predictive of an exudate with a sensitivity and a specificity of 90% and 60%, respectively. Moreover, pleural AAG was significantly higher in cancerous exudates than in exudates and transudates of all other cause taken together (p < 0.001). Finally, to differentiate the same pleural effusion, the cut-off value of 1.0 mg/dl of pleural CRP has a sensitivity and a specificity of 74% and 74%, respectively.

Conclusions: We conclude that both AAG and AMG concentrations in pleural effusions have a high sensitivity and are therefore useful parameters in distinguishing exudates from transudates, but the latter is inferior due to its unacceptably low specificity.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers
  • C-Reactive Protein / analysis*
  • Female
  • Humans
  • Male
  • Middle Aged
  • Orosomucoid / analysis*
  • Pleural Effusion / chemistry*
  • Pleural Effusion / diagnosis*
  • Prospective Studies
  • ROC Curve
  • Sensitivity and Specificity
  • alpha-Macroglobulins / analysis*

Substances

  • Biomarkers
  • Orosomucoid
  • alpha-Macroglobulins
  • C-Reactive Protein