Effects of CTx and 8-bromo-cAMP on LPS-induced gene expression of cytokines in murine peritoneal macrophages

Biochem Biophys Res Commun. 2000 Mar 16;269(2):570-3. doi: 10.1006/bbrc.2000.2341.

Abstract

LPS, an endotoxin isolated from gram-negative bacterial, has been shown to be a potent cytokine initiator in murine peritoneal macrophages. CAMP-dependent pathway is generally considered to play a suppressive role in immune response. This study investigated the effect of cAMP on LPS-induced gene expression of cytokines in murine macrophages. Our data clearly demonstrated that in LPS-treated macrophages, cAMP elevator (CTx and 8-bromo-cAMP) could increase IL-1Ra and IL-10 gene expression, while mRNAs of IL-1alpha, IL-12, IL-6, and MIF were decreased and other cytokines like IL-1beta, and IFN-gamma did not give a definite tendency. This is the first report that CTx and 8-bromo-cAMP positively regulate IL-1Ra gene expression in LPS-stimulated macrophages. Our data also suggest that a cAMP-dependent pathway may play a regulatory role in Toll-receptor system.

MeSH terms

  • 8-Bromo Cyclic Adenosine Monophosphate / pharmacology*
  • Animals
  • Cholera Toxin / pharmacology*
  • Cytokines / genetics*
  • Gene Expression Regulation / drug effects*
  • Lipopolysaccharides / pharmacology*
  • Macrophages, Peritoneal / drug effects*
  • Macrophages, Peritoneal / metabolism
  • Mice

Substances

  • Cytokines
  • Lipopolysaccharides
  • 8-Bromo Cyclic Adenosine Monophosphate
  • Cholera Toxin