Translocation and coamplification of loci from chromosome arms 8p and 11q in the MDA-MB-175 mammary carcinoma cell line

Int J Oncol. 2000 Apr;16(4):683-8. doi: 10.3892/ijo.16.4.683.

Abstract

Rearrangement and coamplification of the 8p12 and 11q13 chromosomal regions occurs in a significant proportion of breast cancers. It usually involves a complex hybrid structure in which the FGFR1 and CCND1 genes are amplified. We report here a different type of 8p12-11q13 rearrangement in the MDA-MB-175 mammary carcinoma cell line. This amplification contains the NRG1/HGL (from 8p12-21) and DOC4 (from 11q13) genes, encoding respectively a ligand for ERBB receptors and a stress-induced protein which is a mammalian ortholog of Drosophila Tenm/Odz. It has been shown previously (Wang et al, Oncogene 18: 5718-5721, 1999) that these two genes are rearranged and fused by a translocation event. This type of event was not found in 30 tumors tested that showed coamplification of the 8p12 and 11q13 regions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms / genetics*
  • Chromosome Mapping
  • Chromosomes, Human, Pair 11*
  • Chromosomes, Human, Pair 8*
  • Female
  • Humans
  • In Situ Hybridization, Fluorescence
  • Membrane Proteins
  • Neuregulin-1 / genetics
  • Nuclear Proteins / genetics
  • Polymerase Chain Reaction
  • Translocation, Genetic*
  • Tumor Cells, Cultured

Substances

  • Membrane Proteins
  • Neuregulin-1
  • Nuclear Proteins
  • Tenm4 protein, mouse