Comparison of non-viral transfection methods in melanoma cell primary cultures

Cytokine. 2000 Jun;12(6):828-33. doi: 10.1006/cyto.1999.0621.

Abstract

Melanoma primary cultures were transiently transfected via electroporation and lipofection for comparison. Transfection efficiency was superior with electroporation (58+/-9%) as compared to lipofection (23+/-9%) as determined by enhanced green fluorescent plasmid (EGFP) transfection. Secretion of IL-2 persisted for up to 3 weeks after electroporation. The increase in sensitivity against immunologic effector cells by transfection with IL-2 was not significant. Our results show the feasibility of a gene transfer into primary human melanoma cells, different from retroviral transduction.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / analysis
  • Cation Exchange Resins
  • Drug Carriers
  • Electroporation / methods
  • Genes, Reporter*
  • Green Fluorescent Proteins
  • Humans
  • Immunophenotyping
  • Interleukin-2 / analysis
  • Interleukin-2 / genetics*
  • Lipids
  • Luminescent Proteins / analysis
  • Luminescent Proteins / genetics
  • Melanoma*
  • Recombinant Proteins / analysis
  • Recombinant Proteins / biosynthesis
  • Transfection / methods*
  • Tumor Cells, Cultured

Substances

  • Antigens, CD
  • Cation Exchange Resins
  • Drug Carriers
  • Interleukin-2
  • Lipids
  • Lipofectamine
  • Luminescent Proteins
  • Recombinant Proteins
  • Green Fluorescent Proteins