Zinc tetraruthenated porphyrin binding and photoinduced oxidation of calf-thymus DNA

J Inorg Biochem. 2000 Mar;78(4):269-73. doi: 10.1016/s0162-0134(00)00053-2.

Abstract

The photooxidation of calf-thymus DNA has been investigated in the presence of a supramolecular tetraruthenated zincporphyrin (ZnTRP) sensitizer. A strong interaction of ZnTRP with DNA has been observed, exhibiting a gradual transition from a non-specific electrostatic binding mode to a more specific one at high DNA concentrations. Formation of O2(1delta(g)) has been detected from its near-infrared emission, after the excitation of ZnTRP in dioxygen-containing solutions. In the presence of DNA and dioxygen, ZnTRP promotes efficient photocatalytic oxidation of the 2'-deoxyguanosine sites, via their direct reaction with O2(1delta(g)), as in a previous work on the ZnTRP-photoinduced oxidation of the free nucleosides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Chromatography, High Pressure Liquid
  • DNA / drug effects
  • DNA / metabolism*
  • DNA / radiation effects
  • Dose-Response Relationship, Drug
  • Kinetics
  • Light
  • Models, Chemical
  • Organometallic Compounds / metabolism*
  • Oxygen / metabolism
  • Porphyrins / metabolism*
  • Protein Binding
  • Spectrometry, Fluorescence
  • Thymus Gland / metabolism*
  • Time Factors
  • Zinc / metabolism*

Substances

  • Organometallic Compounds
  • Porphyrins
  • tetraruthenated porphyrin
  • DNA
  • Zinc
  • Oxygen