Structural organization of the RNA polymerase-promoter open complex

N Naryshkin; A Revyakin; Y Kim; V Mekler; R H Ebright

doi:10.1016/s0092-8674(00)80872-7

Structural organization of the RNA polymerase-promoter open complex

Cell. 2000 Jun 9;101(6):601-11. doi: 10.1016/s0092-8674(00)80872-7.

Authors

N Naryshkin¹, A Revyakin, Y Kim, V Mekler, R H Ebright

Affiliation

¹ Howard Hughes Medical Institute, Department of Chemistry, Rutgers University, Piscataway, New Jersey 08854, USA.

PMID: 10892647
DOI: 10.1016/s0092-8674(00)80872-7

Abstract

We have used systematic site-specific protein-DNA photocrosslinking to define interactions between bacterial RNA polymerase (RNAP) and promoter DNA in the catalytically competent RNAP-promoter open complex (RPo). We have mapped more than 100 distinct crosslinks between individual segments of RNAP subunits and individual phosphates of promoter DNA. The results provide a comprehensive description of protein-DNA interactions in RPo, permit construction of a detailed model for the structure of RPo, and permit analysis of effects of a transcriptional activator on the structure of RPo.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Base Sequence
DNA / chemistry
DNA / genetics*
DNA-Directed RNA Polymerases / chemistry
DNA-Directed RNA Polymerases / genetics*
Molecular Sequence Data
Nucleic Acid Conformation
Promoter Regions, Genetic / genetics*
Protein Conformation
Transcription, Genetic

Substances

DNA
DNA-Directed RNA Polymerases

Grants and funding

GM41376/GM/NIGMS NIH HHS/United States