The genes for small nucleolar RNAs in Trypanosoma brucei are organized in clusters and are transcribed as a polycistronic RNA

Nucleic Acids Res. 2000 Aug 1;28(15):2855-61. doi: 10.1093/nar/28.15.2855.

Abstract

Because the organization of snoRNA genes in vertebrates, plants and yeast is diverse, we investigated the organization of snoRNA genes in a distantly related organism, Trypanosoma brucei. We have characterized the second example of a snoRNA gene cluster that is tandemly repeated in the T.BRUCEI: genome. The genes encoding the box C/D snoRNAs TBR12, TBR6, TBR4 and TBR2 make up the cluster. In a genomic organization unique to trypanosomes, there are at least four clusters of these four snoRNA genes tandemly repeated in the T. BRUCEI: genome. We show for the first time that the genes encoding snoRNAs in both this cluster and the SLA cluster are transcribed in an unusual way as a polycistronic RNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteriophage P1 / genetics
  • Base Sequence
  • Blotting, Southern
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA Restriction Enzymes
  • Genes
  • Molecular Sequence Data
  • Multigene Family
  • Polymerase Chain Reaction
  • RNA, Protozoan / chemistry
  • RNA, Protozoan / genetics
  • RNA, Small Nucleolar / chemistry
  • RNA, Small Nucleolar / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tandem Repeat Sequences
  • Transcription, Genetic
  • Trypanosoma brucei brucei / genetics*

Substances

  • RNA, Protozoan
  • RNA, Small Nucleolar
  • DNA Restriction Enzymes

Associated data

  • GENBANK/AF232064