Application of Prastat ELISA in the determination of anti-HLA specificity for immunized patients awaiting kidney transplant: five years' experience

Transpl Int. 2000:13 Suppl 1:S99-105. doi: 10.1007/s001470050289.

Abstract

Three hundred sixty-five patients who underwent cadaver donor kidney transplantation between 1993 and 1998 were divided into four groups: 40 immunized patients with at least one peak panel-reactive antibody (PRA) value more than 50%, 11 hyperimmunized patients with more than three peak PRA values over 50%, 10 retransplanted patients and 304 control patients. Before transplantation, we ascertained the antibody specificities against individual HLA antigens (Prastat Sangstat ELISA method for HLA typing of first donor, husbands of multiparous women and potential donors against whom candidates gave positive cross-matches); thus, patients underwent transplantation excluding the presence of the HLA antigens previously detected and looking for high HLA (class I and II) compatibility. Actuarial graft survival after 12 months was satisfactory in all groups: 87% immunized, 81% hyperimmunized and 80% retransplanted vs 92% controls. Renal function at the end of the first year was similar and the number of rejection episodes in the first 3 months did not significantly differ.

Publication types

  • Comparative Study

MeSH terms

  • Actuarial Analysis
  • Adult
  • Enzyme-Linked Immunosorbent Assay / methods
  • Erythropoietin / therapeutic use
  • Female
  • Graft Rejection / epidemiology
  • Graft Rejection / immunology*
  • Graft Survival / immunology*
  • HLA Antigens / immunology*
  • Histocompatibility Testing*
  • Humans
  • Immunosuppression Therapy / methods
  • Isoantibodies / blood*
  • Kidney Transplantation / immunology*
  • Kidney Transplantation / physiology
  • Male
  • Recombinant Proteins
  • Reoperation
  • Time Factors
  • Tissue Donors
  • Waiting Lists

Substances

  • HLA Antigens
  • Isoantibodies
  • Recombinant Proteins
  • Erythropoietin