Rapid screening assay for mutagen sensitivity and DNA repair capacity in human peripheral blood lymphocytes

Mutagenesis. 2001 Jan;16(1):25-30. doi: 10.1093/mutage/16.1.25.

Abstract

Individual susceptibility to carcinogens, an important determinant of disease risk, is influenced by host factors such as the ability to repair DNA lesions. In order to identify subjects who are at high risk, we have developed a microgel electrophoresis assay for use in molecular epidemiological studies. The assay was validated in a pilot case-control study: Peripheral blood lymphocytes were collected from 100 patients with lung cancer and 110 control patients without cancer and from the same hospital, and stored at -80 degrees C. After thawing, phytohaemagglutinin-stimulated cells were treated with bleomycin at 20 microg/ml for 30 min and the extent of DNA damage and DNA repair capacity were determined by microgel electrophoresis. Peripheral blood lymphocytes from patients with lung cancer were significantly more sensitive to mutagens than those from controls and showed reduced DNA repair capacity (both P < 0.001). Both endpoints were independent risk factors for smoking-related lung cancer. Repeated analysis of peripheral blood lymphocytes from the same individual demonstrated good reproducibility of the assay. Cryopreservation of the lymphocytes for less than or = 12 months did not significantly affect their sensitivity. Our standardized microgel electrophoresis assay is suitable for determining individual sensitivity to mutagens and DNA repair capacity: it is sensitive and faster than cytogenetic assays, and can be applied to native and cryopreserved peripheral blood lymphocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antimetabolites, Antineoplastic / toxicity
  • Bleomycin / toxicity
  • Case-Control Studies
  • Cells, Cultured
  • DNA Damage / drug effects
  • DNA Damage / genetics
  • DNA Repair* / drug effects
  • Electrophoresis, Agar Gel / methods
  • Female
  • Humans
  • Lung Neoplasms / epidemiology
  • Lung Neoplasms / genetics
  • Lung Neoplasms / metabolism
  • Lymphocytes / drug effects*
  • Lymphocytes / metabolism*
  • Male
  • Middle Aged
  • Mutagenicity Tests / methods*
  • Mutagens / toxicity*
  • Pilot Projects

Substances

  • Antimetabolites, Antineoplastic
  • Mutagens
  • Bleomycin