Altered atypical coupling of gamma-aminobutyrate type A receptor agonist and convulsant binding sites in subunit-deficient mouse lines

Brain Res Mol Brain Res. 2001 Jan 31;86(1-2):179-83. doi: 10.1016/s0169-328x(00)00273-4.

Abstract

We searched for subunit correlations for GABA(A) receptor-associated atypically GABA-insensitive [35S]TBPS binding. The homomeric beta3 subunit receptors could be excluded, as GABA-insensitive [35S]TBPS binding was present in beta3-/- mice. Localization of GABA-insensitive [35S]TBPS binding correlated best with those of delta, alpha4 and alpha6 subunit mRNAs. The amounts of GABA-insensitive [35S]TBPS binding components were increased in delta-/- mice, but dramatically reduced in alpha6-/- mice, suggesting a role for alpha6 but excluding delta subunits.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Binding Sites
  • Brain Chemistry / genetics*
  • Bridged Bicyclo Compounds, Heterocyclic / metabolism
  • Bridged Bicyclo Compounds, Heterocyclic / pharmacology*
  • Convulsants / metabolism
  • Convulsants / pharmacology*
  • GABA Agonists / metabolism
  • GABA Agonists / pharmacology
  • Mice
  • Mice, Knockout
  • Molecular Sequence Data
  • RNA, Messenger / analysis
  • Radioligand Assay
  • Receptors, GABA-A* / chemistry
  • Receptors, GABA-A* / genetics
  • Receptors, GABA-A* / metabolism
  • Sulfur Radioisotopes

Substances

  • Bridged Bicyclo Compounds, Heterocyclic
  • Convulsants
  • GABA Agonists
  • RNA, Messenger
  • Receptors, GABA-A
  • Sulfur Radioisotopes
  • tert-butylbicyclophosphorothionate

Associated data

  • GENBANK/AF189262
  • GENBANK/L08490
  • GENBANK/L08491
  • GENBANK/L08492
  • GENBANK/L08493
  • GENBANK/L08494
  • GENBANK/L08495
  • GENBANK/L08496
  • GENBANK/L08497
  • GENBANK/X15466
  • GENBANK/X15467
  • GENBANK/X15468
  • GENBANK/X57514
  • GENBANK/X63324