Forensic validation of a multiplex containing nine STRs--population genetics in northern Poland

R Pawlowski; A Maciejewska

doi:10.1007/s004140000139

Forensic validation of a multiplex containing nine STRs--population genetics in northern Poland

Int J Legal Med. 2000;114(1-2):45-9. doi: 10.1007/s004140000139.

Authors

R Pawlowski¹, A Maciejewska

Affiliation

¹ Institute of Forensic Medicine, Medical University of Gdansk, 80-210 Gdansk, Debinki 7, Poland. Richard@amg.gda.pl

PMID: 11197627
DOI: 10.1007/s004140000139

Abstract

This paper presents the allele frequency distributions for the nine loci (D3S1358, VWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317 and D7S820) present in the commercially available Profiler Plus kit. DNA samples of 202 individuals from Northern Poland were amplified in a multiplex reaction with subsequent automatic detection using capillary electrophoresis. All loci met the Hardy-Weinberg equilibrium conditions. The calculated probability of identity was 2.26 x 10(-11) giving an average probability of identity of 1 in 44 billion. Considerable improvement of analysis precision was observed after substituting the GeneScan 500 for the fluorescent ladder 60-400 bp. The influence of DNA concentration on stutter and artefact formation and the ratio of heterozygote alleles was analysed.

MeSH terms

DNA Fingerprinting / methods*
Electrophoresis, Capillary
Gene Frequency
Genetics, Population*
Humans
Likelihood Functions
Poland
Predictive Value of Tests
Reproducibility of Results
Tandem Repeat Sequences*