Enzymatic preparation of genuine prosapogenin, 20(S)-ginsenoside Rh1, from ginsenosides Re and Rg1

Biosci Biotechnol Biochem. 2000 Dec;64(12):2739-43. doi: 10.1271/bbb.64.2739.

Abstract

It was found that a lactase preparation from Penicillium sp. nearly quantitatively hydrolyzed ginsenosides Re and Rg1, which are major saponins in roots of Panax ginseng, to a minor saponin, 20(S)-ginsenoside Rh1 [6-O-beta-D-glucopyranosyl-20(S)-protopanaxatriol]. This is the first report on the enzymatic preparation of ginsenoside Rh1 with a high efficiency. This enzyme also readily hydrolyzed ginsenoside Rg2 to ginsenoside Rh1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biochemistry / methods
  • Ginsenosides*
  • Hydrolysis
  • Lactase
  • Saponins / chemical synthesis*
  • Saponins / chemistry*
  • Saponins / metabolism
  • beta-Galactosidase / chemistry*
  • beta-Galactosidase / metabolism

Substances

  • Ginsenosides
  • Saponins
  • ginsenoside Re
  • ginsenoside Rg2
  • ginsenoside Rh1
  • Lactase
  • beta-Galactosidase
  • ginsenoside Rg1