Development of a novel screen for protease inhibitors

Clin Diagn Lab Immunol. 2001 Mar;8(2):437-40. doi: 10.1128/CDLI.8.2.437-440.2001.

Abstract

We have developed a novel plasmid-based, quantitative, in vitro screen to test the protease-inhibiting activities of existing and newly discovered agents.

MeSH terms

  • Acquired Immunodeficiency Syndrome / drug therapy*
  • Drug Evaluation, Preclinical / methods*
  • Genes, Reporter
  • HIV Protease / genetics*
  • HIV Protease Inhibitors / pharmacology*
  • HIV-1 / drug effects
  • HIV-1 / genetics*
  • HeLa Cells
  • Humans
  • Luciferases
  • Plasmids
  • Transfection

Substances

  • HIV Protease Inhibitors
  • Luciferases
  • HIV Protease