Objective: To set up a convenient method for analysis of the amplified fragment length polymorphism (amp-FLP) of two microsatellite DNA in vWF gene.
Methods: Multi-PCR followed by denatured PAGE and silver stain were used to analyze the amp-FLP of two locus (A and B) on one gel. DNA samples from 112 normal people and two vWD families in the Shanghai area were assayed.
Results: 1. seven and 5 types of amp-FLP were identified on loci A and B, respectively. The heterozygote rates were 75% and 74%, respectively. 2. Haplotypes A2/B3 and A4/B3 were found to link with defective vWF genes in the two vWD families, respectively.
Conclusion: 1. Multi-PCR is a fast and practical method to carry out family analysis of inherited diseases. 2. nt1980-1990 and nt2215-2380 of vWF gene are two ideal genetic labels in linkage study and hereditary consultation of vWD family.