Molecular cloning and analysis of the 5'-flanking region of the human MYPT1 gene

Biochim Biophys Acta. 2001 Feb 16;1517(3):424-9. doi: 10.1016/s0167-4781(00)00285-2.

Abstract

We have cloned and sequenced a 5 kb genomic fragment in the 5'-flanking region of the human myosin phosphatase target subunit 1. The transcription initiation site (+1) was 268 bp upstream from the translation start site. In this promoter there are no canonical TATA or CAAT box elements but there is a high GC-rich sequence. Basal promoter activity was due to the GC-rich region that contained one Sp1 transcription factor binding site, thus demonstrating that the MYPT1 gene is a housekeeping gene. Luciferase reporter assays showed the presence of two regions for positive elements and one for a negative element.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Binding Sites
  • Cells, Cultured
  • Cloning, Molecular
  • DNA / genetics
  • DNA / metabolism
  • DNA Primers / genetics
  • HeLa Cells
  • Humans
  • Luciferases / genetics
  • Molecular Sequence Data
  • Myosin-Light-Chain Phosphatase
  • Phosphoprotein Phosphatases / chemistry
  • Phosphoprotein Phosphatases / genetics*
  • Promoter Regions, Genetic
  • Protein Subunits
  • Sp1 Transcription Factor / metabolism
  • Transcription, Genetic

Substances

  • DNA Primers
  • Protein Subunits
  • Sp1 Transcription Factor
  • DNA
  • Luciferases
  • Phosphoprotein Phosphatases
  • Myosin-Light-Chain Phosphatase

Associated data

  • GENBANK/AB042280
  • GENBANK/AB042281