NtcA-dependent expression of the devBCA operon, encoding a heterocyst-specific ATP-binding cassette transporter in Anabaena spp

J Bacteriol. 2001 Jun;183(12):3795-9. doi: 10.1128/JB.183.12.3795-3799.2001.

Abstract

The devBCA operon, encoding subunits of an ATP-binding cassette exporter, is essential for differentiation of N(2)-fixing heterocysts in Anabaena spp. Nitrogen deficiency-dependent transcription of the operon and the use of its transcriptional start point, located 762 (Anabaena variabilis strain ATCC 29413-FD) or 704 (Anabaena sp. strain PCC 7120) bp upstream of the translation start site, were found to require the global nitrogen transcriptional regulator NtcA. Furthermore, NtcA was shown to bind in vitro to the promoter of devBCA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters / genetics*
  • ATP-Binding Cassette Transporters / metabolism
  • Anabaena / genetics*
  • Anabaena / metabolism
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Blotting, Northern
  • DNA, Bacterial / analysis
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Gene Expression Regulation, Bacterial*
  • Nitrogen Fixation
  • Operon
  • Promoter Regions, Genetic
  • RNA, Messenger / analysis
  • Spores, Bacterial
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Transcription, Genetic

Substances

  • ATP-Binding Cassette Transporters
  • Bacterial Proteins
  • DNA, Bacterial
  • DNA-Binding Proteins
  • DevA protein, Anabaena
  • RNA, Messenger
  • Transcription Factors
  • ntcA protein, Synechococcus