Characterization of IS6110 restriction fragment length polymorphism patterns and mechanisms of antimicrobial resistance for multidrug-resistant isolates of Mycobacterium tuberculosis from a major reference hospital in Assiut, Egypt

S Abbadi; H G Rashed; G P Morlock; C L Woodley; O El Shanawy; R C Cooksey

doi:10.1128/JCM.39.6.2330-2334.2001

Characterization of IS6110 restriction fragment length polymorphism patterns and mechanisms of antimicrobial resistance for multidrug-resistant isolates of Mycobacterium tuberculosis from a major reference hospital in Assiut, Egypt

J Clin Microbiol. 2001 Jun;39(6):2330-4. doi: 10.1128/JCM.39.6.2330-2334.2001.

Authors

S Abbadi¹, H G Rashed, G P Morlock, C L Woodley, O El Shanawy, R C Cooksey

Affiliation

¹ Division of AIDS, STD, and TB Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.

Abstract

We evaluated 25 Mycobacterium tuberculosis isolates from patients at a major Egyptian reference hospital in Assiut, Egypt, who had been treated for at least 1 year for tuberculosis. Typing patterns (IS6110) were diverse, and multidrug resistance was found among 11 (44%) of the isolates. Mutations associated with antimicrobial drug resistance were found in rpoB, katG, rpsL, and embB in the resistant isolates.

Publication types

Research Support, U.S. Gov't, P.H.S.

MeSH terms

Antitubercular Agents / pharmacology
Bacterial Proteins / genetics
Bacterial Typing Techniques
DNA Transposable Elements / genetics*
Drug Resistance, Multiple / genetics
Egypt
Humans
Mutation
Mycobacterium tuberculosis / classification*
Mycobacterium tuberculosis / drug effects*
Mycobacterium tuberculosis / genetics
Polymorphism, Restriction Fragment Length
Tuberculosis, Multidrug-Resistant / drug therapy
Tuberculosis, Multidrug-Resistant / microbiology*
Tuberculosis, Pulmonary / drug therapy
Tuberculosis, Pulmonary / microbiology*

Substances

Antitubercular Agents
Bacterial Proteins
DNA Transposable Elements