Level of Flt3-ligand in plasma: a possible new bio-indicator for radiation-induced aplasia

Int J Radiat Biol. 2001 Jun;77(6):703-12. doi: 10.1080/09553000110043711.

Abstract

Purpose: To follow plasma Flt3-ligand (FL) concentrations in irradiated animals in order to evaluate it as an indicator of bone marrow damage for the management of accidental radiation-induced aplasia.

Materials and methods: Non-human primates were irradiated at doses ranging from 2 to 8 Gy, using whole- or partial-body irradiation. Plasma FL concentrations and blood cell counts were determined daily.

Results: FL concentrations increased as early as day 2 after irradiation, whatever the irradiation dose. Increase in plasma FL concentration on day 5 post-irradiation was correlated with radiation dose and with the severity of radiation-induced aplasia. During the course of aplasia, FL concentrations in plasma were inversely correlated with neutrophil counts. A peak in FL concentration appeared before the neutrophil nadir, and the subsequent decrease in FL concentration was correlated with the recovery of blood-cell populations.

Conclusions: Monitoring plasma FL concentration can be used as an indicator of radiation-induced marrow aplasia, and this may be of use in accidental irradiation situations.

MeSH terms

  • Animals
  • Biomarkers / blood
  • Bone Marrow / injuries
  • Bone Marrow / radiation effects
  • Bone Marrow Diseases / blood*
  • Bone Marrow Diseases / etiology
  • Bone Marrow Diseases / therapy
  • Bone Marrow Transplantation
  • Circadian Rhythm
  • Colony-Forming Units Assay
  • Cross Reactions
  • Female
  • Humans
  • Leukocyte Count
  • Macaca fascicularis
  • Male
  • Membrane Proteins / blood*
  • Membrane Proteins / immunology
  • Neutrophils
  • Platelet Count
  • Proto-Oncogene Proteins / immunology
  • Radiation Injuries, Experimental / blood*
  • Radiation Injuries, Experimental / etiology
  • Radiation Injuries, Experimental / therapy
  • Receptor Protein-Tyrosine Kinases / immunology
  • Transplantation, Autologous
  • Whole-Body Irradiation
  • fms-Like Tyrosine Kinase 3

Substances

  • Biomarkers
  • Membrane Proteins
  • Proto-Oncogene Proteins
  • flt3 ligand protein
  • FLT3 protein, human
  • Receptor Protein-Tyrosine Kinases
  • fms-Like Tyrosine Kinase 3