Abstract
The HCN family of ion channel subunits underlies the currents I(f) in heart and I(h) and I(q) in the nervous system. In the present study, we demonstrate that minK-related peptide 1 (MiRP1) is a beta subunit for the HCN family. As such, it enhances protein and current expression as well as accelerating the kinetics of activation. Because MiRP1 also functions as a beta subunit for the cardiac delayed rectifier I(Kr), these results suggest that this peptide may have the unique role of regulating both the inward and outward channels that underlie cardiac pacemaker activity. The full text of this article is available at http://www.circresaha.org.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Blotting, Northern
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Cells, Cultured
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Cyclic Nucleotide-Gated Cation Channels
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Gene Expression
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Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
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Ion Channel Gating / physiology
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Ion Channels / genetics
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Ion Channels / metabolism*
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Membrane Potentials / physiology
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Mice
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Molecular Sequence Data
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Multigene Family
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Muscle Proteins*
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Nerve Tissue Proteins*
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Oocytes / cytology
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Oocytes / metabolism
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Patch-Clamp Techniques
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Potassium Channels / genetics
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Potassium Channels / metabolism*
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Potassium Channels, Voltage-Gated*
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Protein Subunits*
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RNA, Messenger / analysis
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RNA, Messenger / metabolism
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Rabbits
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Rats
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Transfection
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Xenopus laevis
Substances
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Cyclic Nucleotide-Gated Cation Channels
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Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
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Ion Channels
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Muscle Proteins
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Nerve Tissue Proteins
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Potassium Channels
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Potassium Channels, Voltage-Gated
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Protein Subunits
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RNA, Messenger
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potassium channel protein I(sk)