Increased erythritol production in fed-batch cultures of Torula sp. by controlling glucose concentration

J Ind Microbiol Biotechnol. 2001 Apr;26(4):248-52. doi: 10.1038/sj.jim.7000122.

Abstract

The effect of glucose concentration on erythritol production by Torula sp. was investigated. The maximum volumetric productivity of erythritol was obtained at an initial glucose concentration of 300 g l(-1) in batch culture. The volumetric productivity was maximal at a controlled glucose concentration of 225 g l(-1), reducing the lag time of the erythritol production. A fed-batch culture was established with an initial glucose concentration of 300 g l(-1) and with a controlled glucose concentration of 225 g l(-1) in medium containing phytic acid as a phosphate source. In this fed-batch culture, a final erythritol production of 192 g l(-1) was obtained from 400 g l(-1) glucose in 88 h. This corresponded to a volumetric productivity of 2.26 g l(-1) h(-1) and a 48% yield.

MeSH terms

  • Cell Division / drug effects
  • Cryptococcus / classification
  • Cryptococcus / drug effects
  • Cryptococcus / growth & development
  • Cryptococcus / metabolism*
  • Erythritol / biosynthesis*
  • Fermentation
  • Glucose / metabolism*
  • Kinetics
  • Phosphates / metabolism
  • Phytic Acid / metabolism
  • Phytic Acid / pharmacology
  • Time Factors

Substances

  • Phosphates
  • Phytic Acid
  • Glucose
  • Erythritol