Effect of the single major proteic fractions of the liver perchloric extract UK101 on the development of oral tumours in Syrian hamsters

J Oral Pathol Med. 2001 Oct;30(9):532-6. doi: 10.1034/j.1600-0714.2001.300904.x.

Abstract

Squamous cell carcinoma of the oral cavity continues to be a major clinical problem, with about 100,000 new deaths each year worldwide. There is therefore a need to search for new tools to aid oral cancer treatment. We tested the inhibitory activity on chemical carcinogenesis of the three principal protein fractions of about 50, 14, and 8.5 kDa of the mixture UK101 derived from goat liver. These are composed principally of a glycoprotein rich in mannose residues, a protein with analogy to the heat shock protein family, and ubiquitin, respectively. The animal model employed was dimethylbenzanthracene-induced hamster cheek pouch carcinoma. Number of tumours per animal, tumour mass per animal, and proliferating cell nuclear antigen (PCNA) in non-tumour mucosa were quantified: the 14-kDa fraction was the most active; this was also confirmed by testing its corresponding recombinant material. The 50-kDa fraction was inactive, while the ubiquitin showed only low inhibitory activity. It is possible that the technique described and the results obtained could lead to an interesting clinical approach to the treatment of oral cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 9,10-Dimethyl-1,2-benzanthracene
  • Analysis of Variance
  • Animals
  • Anticarcinogenic Agents / therapeutic use*
  • Carcinoma, Squamous Cell / chemically induced
  • Carcinoma, Squamous Cell / prevention & control*
  • Cheek
  • Chemoprevention
  • Chi-Square Distribution
  • Cricetinae
  • Goats
  • Hydrolysis
  • Liver Extracts / chemistry
  • Liver Extracts / therapeutic use*
  • Male
  • Mesocricetus
  • Molecular Weight
  • Mouth Mucosa / metabolism
  • Mouth Neoplasms / chemically induced
  • Mouth Neoplasms / prevention & control*
  • Perchlorates / chemistry
  • Proliferating Cell Nuclear Antigen / biosynthesis
  • Protein Synthesis Inhibitors / therapeutic use*

Substances

  • Anticarcinogenic Agents
  • Liver Extracts
  • Perchlorates
  • Proliferating Cell Nuclear Antigen
  • Protein Synthesis Inhibitors
  • 9,10-Dimethyl-1,2-benzanthracene