Kinetically selective binding of single stranded RNA over DNA by a pyrrolidine-amide oligonucleotide mimic (POM)

Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):1169-72. doi: 10.1081/NCN-100002512.

Abstract

Replacing the sugar-phosphodiester backbone of nucleic acids with a pyrrolidine-amide backbone results in an oligonucleotide mimic POM 1 which binds with high affinity and specificity to complementary DNA and RNA. Unlike other modified oligonucleotides, POM binds much more rapidly to single stranded RNA than DNA.

MeSH terms

  • Amides / chemistry
  • Amides / metabolism
  • DNA / chemistry*
  • DNA / metabolism
  • Hydrogen-Ion Concentration
  • Kinetics
  • Molecular Mimicry
  • Oligonucleotides / chemistry*
  • Oligonucleotides / metabolism
  • Osmolar Concentration
  • Pyrrolidines / chemistry*
  • Pyrrolidines / metabolism
  • RNA / chemistry*
  • RNA / metabolism
  • Substrate Specificity

Substances

  • Amides
  • Oligonucleotides
  • Pyrrolidines
  • RNA
  • DNA