Review: cellular substrates of the eukaryotic chaperonin TRiC/CCT

J Struct Biol. 2001 Aug;135(2):176-84. doi: 10.1006/jsbi.2001.4380.

Abstract

The TCP-1 ring complex (TRiC; also called CCT, for chaperonin containing TCP-1) is a large (approximately 900 kDa) multisubunit complex that mediates protein folding in the eukaryotic cytosol. The physiological substrate spectrum of TRiC is still poorly defined. Genetic and biochemical data show that it is required for the folding of the cytoskeletal proteins actin and tubulin. Recent years have witnessed a steady stream of reports that describe other proteins that require TRiC for proper folding. Furthermore, analysis of the transit of newly synthesized proteins through TRiC in intact cells suggests that the chaperonin contributes to the folding of a distinct subset of cellular proteins. Here we review the current understanding of a role for TRiC in the folding of newly synthesized polypeptides, with a focus on some of the individual proteins that require TRiC.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Eukaryotic Cells / chemistry
  • Humans
  • Intracellular Signaling Peptides and Proteins*
  • Microtubule-Associated Proteins*
  • Nuclear Proteins / metabolism*
  • Nuclear Proteins / physiology
  • Protein Binding
  • Protein Folding*
  • Substrate Specificity
  • Ubiquitin-Protein Ligases
  • t-Complex Genome Region

Substances

  • Intracellular Signaling Peptides and Proteins
  • Microtubule-Associated Proteins
  • Nuclear Proteins
  • PPP1R11 protein, human
  • Ubiquitin-Protein Ligases