Molecular characterization of DDX26, a human DEAD-box RNA helicase, located on chromosome 7p12

Braz J Med Biol Res. 2001 Oct;34(10):1237-45. doi: 10.1590/s0100-879x2001001000002.

Abstract

DEAD-box proteins comprise a family of ATP-dependent RNA helicases involved in several aspects of RNA metabolism. Here we report the characterization of the human DEAD-box RNA helicase DDX26. The gene is composed of 14 exons distributed over an extension of 8,123 bp of genomic sequence and encodes a transcript of 1.8 kb that is expressed in all tissues evaluated. The predicted amino acid sequence shows a high similarity to a yeast DEAD-box RNA helicase (Dbp9b) involved in ribosome biogenesis. The new helicase maps to 7p12, a region of frequent chromosome amplifications in glioblastomas involving the epidermal growth factor receptor (EGFR) gene. Nevertheless, co-amplification of DDX26 with EGFR was not detected in nine tumors analyzed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Candida / genetics
  • Chromosomes, Human, Pair 7 / genetics*
  • Conserved Sequence
  • Drosophila / genetics
  • Gene Amplification
  • Gene Expression
  • Genes, erbB-1
  • Glioblastoma / genetics
  • Humans
  • Molecular Sequence Data
  • RNA Helicases / chemistry*
  • RNA Helicases / genetics*
  • RNA-Binding Proteins
  • Ribosomal Proteins
  • Tumor Cells, Cultured
  • Tumor Suppressor Proteins*
  • Yeasts / genetics

Substances

  • INTS6 protein, human
  • RNA-Binding Proteins
  • Ribosomal Proteins
  • Tumor Suppressor Proteins
  • RNA Helicases

Associated data

  • GENBANK/AF247666