Objective: To investigate the influence of calcium hydroxide to intracellular calcium ion of human pulp cells in vitro.
Methods: The sixth generation of human pulp cells were maintained in RPMI 1640 medium supplemented with Fluo-3 probe and Ca (OH)2 for restimulation. The intracellular calcium ion concentration was detected by laser confocal microscope.
Results: The intracellular calcium ion level in Ca (OH)2 pretreatment group increased after Ca (OH)2 stimulation, while it didn't response to Ca (OH)2 stimulus in group without Ca (OH)2, CaCl2 or NaHCO4 pretreatment.
Conclusion: It is important to pretreat the pulp cells with Ca(OH)2 in order to increase the intracellular calcium.