Cell surface proteoglycan syndecan-1 mediates hepatocyte growth factor binding and promotes Met signaling in multiple myeloma

Blood. 2002 Feb 15;99(4):1405-10. doi: 10.1182/blood.v99.4.1405.

Abstract

Heparan sulfate proteoglycans (HSPGs) play a crucial role in growth regulation by assembling signaling complexes and presenting growth factors to their cognate receptors. Within the immune system, expression of the HSPG syndecan-1 (CD138) is characteristic of terminally differentiated B cells, ie, plasma cells, and their malignant counterpart, multiple myeloma (MM). This study explored the hypothesis that syndecan-1 might promote growth factor signaling and tumor growth in MM. For this purpose, the interaction was studied between syndecan-1 and hepatocyte growth factor (HGF), a putative paracrine and autocrine regulator of MM growth. The study demonstrates that syndecan-1 is capable of binding HGF and that this growth factor is indeed a potent stimulator of MM survival and proliferation. Importantly, the interaction of HGF with heparan sulfate moieties on syndecan-1 strongly promotes HGF-mediated signaling, resulting in enhanced activation of Met, the receptor tyrosine kinase for HGF. Moreover, HGF binding to syndecan-1 promotes activation of the phosphatidylinositol 3-kinase/protein kinase B and RAS/mitogen-activated protein kinase pathways, signaling routes that have been implicated in the regulation of cell survival and proliferation, respectively. These results identify syndecan-1 as a functional coreceptor for HGF that promotes HGF/Met signaling in MM cells, thus suggesting a novel function for syndecan-1 in MM tumorigenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Division / drug effects
  • Enzyme Activation
  • Hepatocyte Growth Factor / metabolism*
  • Humans
  • MAP Kinase Signaling System
  • Membrane Glycoproteins / metabolism
  • Membrane Glycoproteins / pharmacology*
  • Membrane Glycoproteins / physiology
  • Multiple Myeloma / etiology
  • Multiple Myeloma / metabolism*
  • Multiple Myeloma / pathology
  • Phosphatidylinositol 3-Kinases / metabolism
  • Protein Binding
  • Protein Serine-Threonine Kinases*
  • Proteoglycans / metabolism
  • Proteoglycans / pharmacology*
  • Proteoglycans / physiology
  • Proto-Oncogene Proteins / metabolism
  • Proto-Oncogene Proteins c-akt
  • Proto-Oncogene Proteins c-met / genetics
  • Proto-Oncogene Proteins c-met / metabolism
  • Proto-Oncogene Proteins c-met / physiology*
  • Signal Transduction / drug effects*
  • Syndecan-1
  • Syndecans
  • Transfection
  • Tumor Cells, Cultured
  • ras Proteins / metabolism

Substances

  • Membrane Glycoproteins
  • Proteoglycans
  • Proto-Oncogene Proteins
  • SDC1 protein, human
  • Syndecan-1
  • Syndecans
  • Hepatocyte Growth Factor
  • Proto-Oncogene Proteins c-met
  • Protein Serine-Threonine Kinases
  • Proto-Oncogene Proteins c-akt
  • ras Proteins