Immuno-capture PCR for detection of Aeromonas hydrophila

Xuanxian Peng; Jianying Zhang; Sanying Wang; Zhilin Lin; Weiyi Zhang

doi:10.1016/s0167-7012(02)00010-6

Immuno-capture PCR for detection of Aeromonas hydrophila

J Microbiol Methods. 2002 May;49(3):335-8. doi: 10.1016/s0167-7012(02)00010-6.

Authors

Xuanxian Peng¹, Jianying Zhang, Sanying Wang, Zhilin Lin, Weiyi Zhang

Affiliation

¹ Department of Biology and the Key Laboratory of Education Ministry for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, PR China. wangpeng@xmu.edu.cn

PMID: 11869801
DOI: 10.1016/s0167-7012(02)00010-6

Abstract

In this report, we describe the use of universal primer PCR (UPPCR) for the detection of 16S ribosomal RNA (rRNA) genes from Aeromonas hydrophila captured by anti-A. hydrophila antibody coupled to a microplate. The approach combining immuno-capture with UPPCR provides a quick, sensitive, and reproducible way for the detection of bacterial cells.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Aeromonas hydrophila / genetics
Aeromonas hydrophila / immunology
Aeromonas hydrophila / isolation & purification*
Antibodies, Bacterial
DNA Primers
Immunoassay
Polymerase Chain Reaction / methods*

Substances

Antibodies, Bacterial
DNA Primers