Abstract
We have previously shown that electromagnetic field (EMF) exposure induces ETS1 oncogene overexpression in different cell lines. In order to investigate in vivo EMF effects, BALB/c mice were exposed at different times to 50 MHz radiation, modulated (80%) at 16 Hz. The exposed and control animals were sacrificed and the spleen excised for rt-pcr and western blot analysis. We observed an increase in ETS1 mRNA and protein expression, but a decrease in ETS2 protein levels. Preliminary results from this experimental model show in vivo evidence of the effect of EMF on ETS oncogene expression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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DNA-Binding Proteins*
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Electromagnetic Fields / adverse effects*
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Gene Expression Regulation*
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Male
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Mice
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Mice, Inbred BALB C
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Proto-Oncogene Protein c-ets-1
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Proto-Oncogene Protein c-ets-2
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins c-ets
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RNA, Messenger
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Repressor Proteins*
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Trans-Activators / genetics*
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Transcription Factors / genetics*
Substances
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DNA-Binding Proteins
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ERF protein, human
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ETS1 protein, human
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Ets1 protein, mouse
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Ets2 protein, mouse
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Proto-Oncogene Protein c-ets-1
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Proto-Oncogene Protein c-ets-2
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-ets
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RNA, Messenger
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Repressor Proteins
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Trans-Activators
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Transcription Factors