Interaction of proteins in solution from small-angle scattering: a perturbative approach

Biophys J. 2002 Apr;82(4):2165-75. doi: 10.1016/S0006-3495(02)75563-X.

Abstract

In this work an improved methodology for studying interactions of proteins in solution by small-angle scattering is presented. Unlike the most common approach, where the protein-protein correlation functions g(ij)(r) are approximated by their zero-density limit (i.e., the Boltzmann factor), we propose a more accurate representation of g(ij)(r) that takes into account terms up to the first order in the density expansion of the mean-force potential. This improvement is expected to be particularly effective in the case of strong protein-protein interactions at intermediate concentrations. The method is applied to analyze small-angle x-ray scattering data obtained as a function of the ionic strength (from 7 to 507 mM) from acidic solutions of beta-lactoglobulin at the fixed concentration of 10 gl(-1). The results are compared with those obtained using the zero-density approximation and show significant improvement, particularly in the more demanding case of low ionic strength.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biophysics / methods
  • Lactoglobulins / chemistry
  • Milk / chemistry
  • Models, Statistical
  • Monte Carlo Method
  • Protein Binding
  • Proteins / chemistry*
  • Scattering, Radiation
  • Spectrophotometry
  • Thermodynamics
  • X-Rays

Substances

  • Lactoglobulins
  • Proteins