Magnetization studies of the active and fluoride-inhibited derivatives of the reduced catalase of Lactobacillus plantarum: toward a general picture of the anion-inhibited and active forms of the reduced dimanganese catalases

J Biol Inorg Chem. 2002 Apr;7(4-5):445-50. doi: 10.1007/s00775-001-0319-x. Epub 2002 Jan 8.

Abstract

The magnetic properties of the reduced catalase from Lactobacillus plantarum have been studied for the active enzyme and its fluoride complex through variable field/variable temperature magnetization measurements. The magnetic exchange interaction deduced from these experiments [fluoride complex: - J=1.3(1) cm(-1); active enzyme: - J=5.6(5) cm(-1); H=-2 J S(1) S(2)] are similar to those presently obtained in a re-analysis of the data for the corresponding forms of the Thermus thermophilus enzyme (previously published in 1997, Angew Chem Int Ed Engl 36:1626-1628): phosphate complex: - J=2.1(2) cm(-1); active enzyme - J=5.0(3) cm(-1). These results concur to a unified picture for the two enzymes, consistent with the presence of a hydroxide bridge in the reduced active catalases and its replacement by an aqua bridge in the anion-inhibited enzymes as the main mediators of the magnetic exchange.

MeSH terms

  • Anions
  • Catalase / antagonists & inhibitors
  • Catalase / chemistry*
  • Catalase / metabolism*
  • Fluorides / pharmacology*
  • Lactobacillus / enzymology*
  • Magnetics
  • Oxidation-Reduction

Substances

  • Anions
  • Catalase
  • Fluorides