Immunocytochemical and ultrastructural characterization of endocrine cells in the larval stomach of the frog Rana temporaria tadpoles: a comparison with adult specimens

Tissue Cell. 2001 Oct;33(5):462-77. doi: 10.1054/tice.2001.0200.

Abstract

According to immunostaining and ultrastructural patterns, Rana temporaria tadpole stomach displays a well-differentiated endocrine population comprising, at least, six cellular types: ECL, EC [serotonin], D [somatostatin] - all three of them abundant -, P [bombesin] - less numerous -, CCK-8 [cholecystokinin/gastrin] and A [glucagon/glicentin] - both very scarce. Larval endocrine cells are mainly located in the surface epithelium and show open or closed morphologies. Cellular diversity is similar in tadpoles and frogs, with the exception of immunoreactivity for gastrin-17, found in adults in numerous cells. Larval cells display mature ultrastructural traits, although with smaller secretory granules. The different distribution of endocrine cells, which in adults are preferentially located in the glands, probably refers to different functional requirements. However, the rich vascular plexus present in larval mucosa may be an efficient transport medium of surface hormones to-gastric targets. The enhancement in adults of endocrine population and correlative increase in hormonal secretion indicates a more active functional role, probably related to the shift from herbivorous to carnivorous habits. In summary, the tadpole gastric endocrine population, although not as numerous as that of adult frogs, displays histological traits that indicate a relevant (immunoreactive and ultrastructural properties, cellular diversity) and specific (surface location, relative abundance of open-type cells) role of local regulatory factors in amphibian larval gastric function.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bombesin / analysis
  • Digestive System / chemistry
  • Digestive System / cytology
  • Glicentin
  • Glucagon / analysis
  • Glucagon-Like Peptides
  • Immunohistochemistry / methods
  • Microscopy, Electron
  • Peptide Fragments / analysis
  • Protein Precursors / analysis
  • Rana temporaria*
  • Serotonin / analysis
  • Silver Staining
  • Sincalide / analysis
  • Somatostatin / analysis
  • Stomach / chemistry
  • Stomach / cytology*

Substances

  • Peptide Fragments
  • Protein Precursors
  • Serotonin
  • Somatostatin
  • Glucagon-Like Peptides
  • Glicentin
  • Glucagon
  • Sincalide
  • Bombesin