Molecular diagnosis of Granulicatella elegans on the cardiac valve of a patient with culture-negative endocarditis

J Clin Microbiol. 2002 May;40(5):1845-7. doi: 10.1128/JCM.40.5.1845-1847.2002.

Abstract

We herein report one case of culture-negative infectious endocarditis (IE) where the organism, Granulicatella elegans, was identified by molecular analysis using broad-range PCR primers complementary to the 16S rRNA gene on the removed valve. The results and utility of this method are discussed.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • DNA Primers
  • Endocarditis, Bacterial / diagnosis*
  • Gram-Positive Bacterial Infections / diagnosis*
  • Humans
  • Male
  • Nucleic Acid Hybridization
  • Polymerase Chain Reaction / methods
  • RNA, Ribosomal, 16S / genetics
  • Streptococcaceae / classification
  • Streptococcaceae / genetics
  • Streptococcaceae / isolation & purification*
  • Tricuspid Valve / microbiology*

Substances

  • DNA Primers
  • RNA, Ribosomal, 16S