Evidence of common progenitors and patterns of dispersion in rat striatum and cerebral cortex

J Neurosci. 2002 May 15;22(10):4002-14. doi: 10.1523/JNEUROSCI.22-10-04002.2002.

Abstract

To correlate clonal patterns in the rat striatum with adult neuronal phenotypes, we labeled striatal progenitors between embryonic day 14 (E14) and E19 with a retroviral library encoding alkaline phosphatase. In the adult striatum, the majority of E14-labeled neurons (87%) were members of discrete horizontal or radial cell clusters. Radial clusters accounted for only 23% of cell clusters but >34% of labeled cells. Striatal clones also demonstrated an unexpected widespread pattern of clonal dispersion. The majority of striatal clones were widely dispersed within the striatum, and 80% of clones were part of even larger clones that included cortical interneurons. Finally, we observed that PCR-positive cortical interneurons were members of clones containing both interneurons and pyramids (44%), exclusively interneuron clones (24%), or combined striatal-cortical clones (16%), consistent with the view that cortical interneurons have multiple origins in differentially behaving progenitor cells. Our data are also consistent with the notion that similar mechanisms underpin striatal and cortical development.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alkaline Phosphatase / analysis
  • Alkaline Phosphatase / biosynthesis
  • Alkaline Phosphatase / genetics
  • Animals
  • Cell Lineage
  • Cell Movement / physiology
  • Cerebral Cortex / cytology*
  • Cerebral Cortex / embryology
  • Cerebral Cortex / enzymology
  • Clone Cells / cytology*
  • Clone Cells / enzymology
  • Cluster Analysis
  • Corpus Striatum / cytology*
  • Corpus Striatum / embryology
  • Corpus Striatum / enzymology
  • Fetus
  • Genetic Vectors / administration & dosage
  • Genetic Vectors / genetics
  • Genetic Vectors / metabolism
  • Gestational Age
  • Lateral Ventricles
  • Lysine / analogs & derivatives*
  • Microinjections
  • Neuroglia / cytology
  • Neuroglia / enzymology
  • Neurons / cytology*
  • Neurons / enzymology
  • Phenotype
  • Polymerase Chain Reaction
  • Rats
  • Rats, Long-Evans
  • Retroviridae / genetics
  • Stem Cells / cytology*
  • Stem Cells / enzymology

Substances

  • Alkaline Phosphatase
  • biocytin
  • Lysine