Lysophosphatidic acid inhibition of the accumulation of Pseudomonas aeruginosa PAO1 alginate, pyoverdin, elastase and LasA

Microbiology (Reading). 2002 Jun;148(Pt 6):1709-1723. doi: 10.1099/00221287-148-6-1709.

Abstract

The pathogenesis of Pseudomonas aeruginosa is at least partially attributable to its ability to synthesize and secrete the siderophore pyoverdin and the two zinc metalloproteases elastase and LasA, and its ability to form biofilms in which bacterial cells are embedded in an alginate matrix. In the present study, a lysophospholipid, 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphate [also called monopalmitoylphosphatidic acid (MPPA)], which accumulates in inflammatory exudates, was shown to inhibit the extracellular accumulation of P. aeruginosa PAO1 alginate, elastase, LasA protease and the siderophore pyoverdin. MPPA also inhibited biofilm formation. The inhibitory effects of MPPA occur independently of rpoS expression and without affecting the accumulation of the autoinducers N-(3-oxododecanoyl) homoserine lactone and N-butyryl-L-homoserine lactone, and may be due, at least in part, to the ability of MPPA to bind divalent cations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alginates / metabolism*
  • Bacterial Proteins / biosynthesis
  • Bacterial Proteins / genetics
  • Biofilms / drug effects
  • Biofilms / growth & development
  • Edetic Acid / pharmacology
  • Enzyme Activation / drug effects
  • Exopeptidases / biosynthesis
  • Exopeptidases / metabolism
  • Glucuronic Acid
  • Hexuronic Acids
  • Lysophospholipids / pharmacology*
  • Metalloendopeptidases / biosynthesis
  • Metalloendopeptidases / metabolism*
  • Metals / pharmacology
  • O Antigens / biosynthesis
  • O Antigens / metabolism
  • Oligopeptides*
  • Pigments, Biological / metabolism*
  • Pseudomonas aeruginosa / drug effects*
  • Pseudomonas aeruginosa / enzymology
  • Pseudomonas aeruginosa / growth & development
  • Pseudomonas aeruginosa / metabolism*
  • Siderophores / metabolism
  • Sigma Factor / biosynthesis
  • Sigma Factor / genetics

Substances

  • Alginates
  • Bacterial Proteins
  • Hexuronic Acids
  • Lysophospholipids
  • Metals
  • O Antigens
  • Oligopeptides
  • Pigments, Biological
  • Siderophores
  • Sigma Factor
  • sigma factor KatF protein, Bacteria
  • pyoverdin
  • Glucuronic Acid
  • Edetic Acid
  • Exopeptidases
  • Metalloendopeptidases
  • staphylolytic protease