Interleukin-10 promoter polymorphisms and cervical cancer risk in Korean women

Cancer Lett. 2002 Oct 8;184(1):57-63. doi: 10.1016/s0304-3835(02)00193-3.

Abstract

The aims of this study were to determine whether polymorphisms of the interleukin (IL)-10 promoter might be associated with an increased risk of cervical cancer, and further whether systemic IL-10 concentration might be influenced by the genotypes in Korean women. Peripheral blood samples from patients with invasive cervical cancer (ICC, n=144) and non-cancer controls (NCC, n=179) were used to detect three biallelic IL-10 promoter polymorphisms at -1082, -819, and -592 sites by polymerase chain reaction-restriction fragment length polymorphism assay using MnlI, MaeIII and RsaI, respectively. The IL-10 serum concentration was measured with enzyme-linked immunosorbent assay. We compared the distribution of genotypes in both groups, evaluated the serum IL-10 level according to the genotypes, and analyzed the association of these polymorphisms with the risk of cervical cancer. The genotype at the -1082 site exhibited only the *A homozygote, and only two haplotypes were found in Korean women, one being -1082*A/-819*T/-592*A (ATA) and the other -1082*A/-819*C/-592*C (ACC). No association was found for IL-10 promoter polymorphisms among the ICC patients in comparison with the NCC subjects. The risk of cervical cancer was not increased in either genotype and the IL-10 serum concentration was not influenced by the genotypes in either group. Polymorphisms of the IL-10 promoter do not appear to be associated with cervical cancer risk or systemic IL-10 production in Korean women.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Case-Control Studies
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Genetic Markers
  • Genotype
  • Homozygote
  • Humans
  • Interleukin-10 / blood
  • Interleukin-10 / genetics*
  • Korea / epidemiology
  • Neoplasm Invasiveness / genetics*
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*
  • Polymorphism, Restriction Fragment Length
  • Promoter Regions, Genetic / genetics*
  • Risk Factors
  • Uterine Cervical Neoplasms / blood
  • Uterine Cervical Neoplasms / genetics*
  • Women's Health

Substances

  • Genetic Markers
  • Interleukin-10