We used a screening protocol to identify Staphylococcus lugdunensis in clinical specimens with pure or predominant growth of coagulase-negative staphylococci. S. lugdunensis was isolated from 17 patients in a 12-month period and was judged to be the cause of infection in 14 cases. In 13 cases, the patient had a soft-tissue abscess, and in 9 of these, the abscess was located in the pelvic girdle region, which suggests that this may be the natural habitat of S. lugdunensis.