Expression patterns of antioxidant proteins in brains of patients with sporadic Creutzfeldt-Jacob disease

Electrophoresis. 2002 Aug;23(15):2541-7. doi: 10.1002/1522-2683(200208)23:15<2541::AID-ELPS2541>3.0.CO;2-1.

Abstract

Using two-dimensional gel electrophoresis (2-DE) and Western blot analysis, we were able to identify and quantify six antioxidant proteins, peroxiredoxin (Prx) I, Prx II, Prx III, 1-Cys Prx, putative peroxisomal antioxidant enzyme (PLP), and mitochondrial Mn superoxide dismutase (Mn-SOD) in two individual brain regions, cerebellum and frontal cortex of patients with sporadic Creutzfeldt-Jacob (sCJD). Among six antioxidant proteins, 1-Cys Prx showed significant increase (P > 0.05) in sCJD frontal cortex whereas Prx I was decreased (P > 0.01). In cerebellum, levels of all antioxidant proteins studied were comparable to those of controls. Our findings provide evidence for the link between aberrant expression of antioxidant proteins, 1-Cys Prx and Prx I and CJD neuropathogenesis and we discuss the neuropathological meaning of these dysregulated antioxidant proteins in sCJD brain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Antioxidants / isolation & purification
  • Blotting, Western
  • Case-Control Studies
  • Creutzfeldt-Jakob Syndrome / genetics
  • Creutzfeldt-Jakob Syndrome / metabolism*
  • Electrophoresis, Gel, Two-Dimensional / methods*
  • Female
  • Gene Expression
  • Humans
  • Male
  • Middle Aged
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / isolation & purification*
  • Peroxidases / genetics
  • Peroxidases / isolation & purification
  • Peroxiredoxins
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Antioxidants
  • Nerve Tissue Proteins
  • Peroxidases
  • Peroxiredoxins