Abstract
We have synthesized and evaluated the activity of 3,6-disubstituted pyrazolo[1,5-a]pyrimidines as a new class of KDR kinase inhibitors. Starting with screening lead 1, potency against isolated KDR was fully optimized with 3-thienyl and 4-methoxyphenyl substituents at the 6- and 3-positions (3g, KDR IC(50)=19 nM), respectively. The synthesis and SAR of these compounds are described.
MeSH terms
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Endothelial Growth Factors / antagonists & inhibitors
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Endothelial Growth Factors / pharmacology
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Endothelium, Vascular / cytology
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Endothelium, Vascular / drug effects
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Enzyme Inhibitors / chemical synthesis*
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Enzyme Inhibitors / pharmacology*
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Humans
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Intercellular Signaling Peptides and Proteins / pharmacology
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Lymphokines / antagonists & inhibitors
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Lymphokines / pharmacology
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Mitogens / antagonists & inhibitors
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Mitogens / pharmacology
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Pyrazoles / chemical synthesis*
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Pyrazoles / pharmacology*
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Pyrimidines / chemical synthesis*
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Pyrimidines / pharmacology*
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Structure-Activity Relationship
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factor Receptor-2 / antagonists & inhibitors*
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Vascular Endothelial Growth Factors
Substances
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Endothelial Growth Factors
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Enzyme Inhibitors
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Intercellular Signaling Peptides and Proteins
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Lymphokines
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Mitogens
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Pyrazoles
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Pyrimidines
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factors
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Vascular Endothelial Growth Factor Receptor-2