Abstract
Extensive biochemical testing and 16S rRNA and rpoB sequence analysis revealed that clinical strain CF01Ent1, initially identified as Buttiauxella agrestis by the use of Api 32 biochemical strips, is a new organism in the Enterobacteriaceae family. It produced an inducible AmpC-type beta-lactamase whose sequence shares 69 to 72% identity with those of the other AmpC-type beta-lactamases of ENTEROBACTERIACEAE: This enzyme exhibits an atypical high affinity for all beta-lactams tested.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics
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Base Sequence
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DNA-Directed RNA Polymerases / genetics
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Enterobacteriaceae / classification*
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Enterobacteriaceae / drug effects
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Enterobacteriaceae / enzymology*
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Enterobacteriaceae / genetics
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Enzyme Induction
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Microbial Sensitivity Tests
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Molecular Sequence Data
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
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beta-Lactam Resistance
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beta-Lactamases / biosynthesis*
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beta-Lactamases / chemistry
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beta-Lactamases / genetics
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beta-Lactams / pharmacology
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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RNA, Ribosomal, 16S
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beta-Lactams
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AmpR protein, Bacteria
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DNA-Directed RNA Polymerases
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RNA polymerase beta subunit
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AmpC beta-lactamases
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beta-Lactamases
Associated data
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GENBANK/AF440406
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GENBANK/AJ489826
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GENBANK/AJ489827