A new approach to oligonucleotide labelling using Diels-Alder cycloadditions and detection by SERRS

Chem Commun (Camb). 2002 Sep 21:(18):2100-1. doi: 10.1039/b204790j.

Abstract

Diels-Alder cycloaddition has been used to add a benzotriazole azo dye to a diene tagged oligonucleotide to generate unique SERRS signals at 10 attomoles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Azo Compounds / chemistry
  • Coloring Agents / chemistry*
  • Cyclization
  • DNA / analysis
  • Furans / chemistry
  • Maleimides / chemistry
  • Oligonucleotide Probes / chemical synthesis*
  • Oligonucleotide Probes / chemistry
  • Oligonucleotides / chemistry*
  • Scattering, Radiation
  • Spectrum Analysis, Raman / methods*
  • Surface Properties
  • Triazoles / chemistry

Substances

  • Azo Compounds
  • Coloring Agents
  • Furans
  • Maleimides
  • Oligonucleotide Probes
  • Oligonucleotides
  • Triazoles
  • maleimide
  • benzotriazole
  • DNA