GENOTRACE: cDNA-based local GENOme assembly from TRACE archives

Bioinformatics. 2002 Oct;18(10):1396-7. doi: 10.1093/bioinformatics/18.10.1396.

Abstract

GENOTRACE identifies the genomic organization for a cDNA using raw data from genome sequencing projects in progress (trace archives). Local genomic contigs are generated, allowing for example the design of PCR primers in intronic sequences to amplify coding regions of a gene, needed for example for mutation or SNP detection.

Availability: The package and examples of output files can be downloaded from http://rat.niob.knaw.nl/GENOTRACE

MeSH terms

  • Archives
  • Chromosome Mapping / methods
  • DNA, Complementary / genetics*
  • Database Management Systems
  • Databases, Nucleic Acid*
  • Hypermedia
  • Information Storage and Retrieval / methods*
  • Internet
  • National Library of Medicine (U.S.)
  • Sequence Analysis, DNA / methods
  • Software Design
  • Software*
  • United States

Substances

  • DNA, Complementary