A dual-expression vector allowing expression in E. coli and P. pastoris, including new modifications

Methods Mol Biol. 2003:205:31-42. doi: 10.1385/1-59259-301-1:31.
No abstract available

MeSH terms

  • Bacteriological Techniques
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers / genetics
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Gene Expression
  • Genetic Vectors*
  • Mycology / methods
  • Pichia / genetics*
  • Pichia / metabolism
  • Plasmids / genetics
  • Protein Denaturation
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification
  • Transformation, Genetic

Substances

  • DNA Primers
  • Recombinant Proteins