The problem of peak identification in crossed immunoelectrophoresis: a modification of the technique of Platt et al

J Clin Chem Clin Biochem. 1976 Feb;14(2):79-81. doi: 10.1515/cclm.1976.14.1-12.79.

Abstract

After a brief review of the known techniques for the identification of crossed immunoelectrophoresis peaks, the authors claim that the technique of Platt et al (1973), Clin. Chim. Acta 46, 419-429) is the most effective. This consists of blocking a protein in the first run, thereby causing the disappearance of the corresponding peak in the second dimension. The advantages of this technique with respect to the others are discussed.

MeSH terms

  • Humans
  • Immune Sera
  • Immunoelectrophoresis / methods*
  • Immunoelectrophoresis, Two-Dimensional / methods*

Substances

  • Immune Sera