Drug targeting Mycobacterium tuberculosis cell wall synthesis: development of a microtiter plate-based screen for UDP-galactopyranose mutase and identification of an inhibitor from a uridine-based library

Antimicrob Agents Chemother. 2003 Jan;47(1):378-82. doi: 10.1128/AAC.47.1.378-382.2003.

Abstract

A microtiter plate assay for UDP-galactopyranose mutase, an essential cell wall biosynthetic enzyme of Mycobacterium tuberculosis, was developed. The assay is based on the release of tritiated formaldehyde from UDP-galactofuranose but not UDP-galactopyranose by periodate and was used to identify a uridine-based enzyme inhibitor from a chemical library.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Wall / enzymology
  • Enzyme Inhibitors / chemical synthesis*
  • Enzyme Inhibitors / pharmacology
  • Intramolecular Transferases / antagonists & inhibitors*
  • Intramolecular Transferases / metabolism
  • Mycobacterium tuberculosis / drug effects
  • Mycobacterium tuberculosis / enzymology*
  • Structure-Activity Relationship

Substances

  • Enzyme Inhibitors
  • Intramolecular Transferases
  • UDP-galactopyranose mutase