Molecular identification of Paracoccidioides brasiliensis by 5' nuclease assay

Diagn Microbiol Infect Dis. 2002 Dec;44(4):383-6. doi: 10.1016/s0732-8893(02)00472-8.

Abstract

A PCR assay based on the 5' nuclease assay using a fluorescent probe derived from the sequence of the gene coding for the 43,000-Da (gp43) antigen was developed to detect Paracoccidioides brasiliensis. The assay could detect at least 10 copies of this DNA sequence, providing efficient accuracy to be useful for diagnosis of paracoccidioidomycosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Neoplasm / genetics
  • DNA Probes
  • Exonucleases / metabolism*
  • Fluorescence
  • Genes, Fungal / genetics
  • Mycological Typing Techniques / methods*
  • Paracoccidioides / genetics*
  • Paracoccidioides / isolation & purification*
  • Polymerase Chain Reaction / methods*

Substances

  • Antigens, Neoplasm
  • DNA Probes
  • tumor-associated antigen, gp43
  • Exonucleases