Pigments of three species of alkaliphilic heliobacteria of the genus Heliorestis, H. daurensis, H. baculata and an undescribed species Heliorestis strain HH, were identified using spectroscopic methods. In these species, bacteriochlorophyll g esterified with farnesol was present, as for other heliobacteria. The carotenoids consisted of 4,4'-diaponeurosporene, also found in other heliobacteria, plus the novel pigments OH-diaponeurosporene glucoside esters (C16:0 and C16:1). In addition, trace amounts of biosynthetic intermediates, OH-diaponeurosporene and OH-diaponeurosporene glucoside, were found. Trace amounts of a carotenoid with 20 carbon atoms, 8,8'-diapo-zeta-carotene, were also found in these species as well as in the non-alkaliphilic heliobacteria. The non-alkaliphilic species Heliophilum fasciatum also contained trace amounts of the two OH-diaponeurosporene glucoside esters. The results are used to predict the pathway of carotenoid biosynthesis in heliobacteria.